Paraprotein Interferences: Insights from a Short Study Involving Multiple Platforms and Multiple Measurands

Area of Science:

• Clinical Chemistry
• Laboratory Medicine
• Analytical Biochemistry

Background:

• Paraprotein interference is a known issue in clinical chemistry.
• Large-scale studies evaluating paraprotein interference across multiple platforms and measurands are lacking.
• This study addresses the need for predictive analysis of paraprotein interference.

Purpose of the Study:

• To evaluate paraprotein interference on different chemistry platforms.
• To compare results between dry and wet chemistry methods.
• To provide predictive analysis of interference patterns.

Main Methods:

• Cross-sectional, non-interventional observational study.
• Thirteen paraproteinaemic subjects analyzed.
• Measurements performed on dry chemistry (Vitros 350) and wet chemistry (AU5800, Cobas 6000) platforms.
• Analyzed total bilirubin, direct bilirubin, HDL-cholesterol, calcium, inorganic phosphate, iron, and unsaturated iron binding capacity (UIBC).

Main Results:

• Significant variations observed for direct bilirubin, HDL, and iron on AU5800 compared to dry chemistry.
• Discrepant results for direct bilirubin and iron noted on Cobas 6000.
• Unsaturated iron binding capacity (UIBC) measurements varied significantly with paraprotein levels on AU5800.

Conclusions:

• Paraprotein interference is common on wet chemistry platforms.
• Predictive analysis indicates frequent interference across multiple measurands.
• Developing algorithms to monitor reaction curves can mitigate erroneous results.