Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photochemical Electrocyclic Reactions: Stereochemistry01:26

Photochemical Electrocyclic Reactions: Stereochemistry

The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Methods of Medium Optimization01:28

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution LC-MS/MS analysis of brain N-glycans reveals composition-specific changes in Parkinson's disease.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026
Same author

Lessons From a Simulation Study Assessing Social Biases of Generative Artificial Intelligence.

The Journal of the Association of Nurses in AIDS Care : JANAC·2026
Same author

Short-Term Fixes, Long-Term Gaps: Addressing Rural Health Workforce Challenges in Queensland.

The Australian journal of rural health·2026
Same author

Redesign of energetically frustrated regions rescues function in defective T4 clamp loaders.

bioRxiv : the preprint server for biology·2026
Same author

DM: a simple solution to suppress air-water interface interactions in cryo-EM.

bioRxiv : the preprint server for biology·2026
Same author

Caring for Australians and New Zealanders with kidney Impairment guidelines commentary on the Kidney Disease: Improving Global Outcomes clinical practice guideline for management of diabetes and chronic kidney disease.

Internal medicine journal·2026

Related Experiment Video

Updated: Jun 25, 2026

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

15.0K

Identification of Tunable, Environmentally Responsive Fluorogenic Dyes by High-Throughput Screening.

Shaochen You1, Thu Nguyen1, Chloris Li-Ma1

  • 1Department of Chemistry, Scripps Research, San Diego, California 92037, United States.

ACS Chemical Biology
|September 9, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed new fluorescent small molecules to detect proteins. These novel fluorogenic probes offer improved performance for characterizing protein states in biological research.

More Related Videos

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

32.8K
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

10.3K

Related Experiment Videos

Last Updated: Jun 25, 2026

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

15.0K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

32.8K
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

10.3K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Molecular Biology

Background:

  • Small molecule dyes are crucial biological tools.
  • Limited availability of environmentally responsive fluorogenic small molecules for protein state characterization.
  • Need for novel probes to study protein lipophilic sites.

Purpose of the Study:

  • To develop and screen for small molecules that exhibit increased fluorescence upon binding to lipophilic protein sites.
  • To identify novel fluorogenic probes for protein characterization.
  • To establish a framework for discovering new fluorophores.

Main Methods:

  • High-throughput screening of small molecules.
  • Assays to detect fluorescence changes upon protein binding.
  • Differential scanning fluorimetry experiments.
  • Structure-activity relationship studies.

Main Results:

  • Identified two small molecules with potent fluorescence response to common proteins.
  • These novel probes outperform existing dyes in differential scanning fluorimetry.
  • Demonstrated tunability of quantum yields and emission wavelengths for the identified scaffolds.

Conclusions:

  • Developed a straightforward framework for discovering new fluorophores.
  • Added two novel fluorogenic probes to the toolkit for protein state analysis.
  • These probes enhance the ability to study protein lipophilic interactions and states.