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Related Concept Videos

Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

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Pericardium decellularization in a one-day, two-step protocol.

P López-Chicón1,2, J I Rodríguez Martínez1,2, C Castells-Sala3,4

  • 1Barcelona Tissue Bank, Banc de Sang i Teixits (BST, GenCAT), Passeig Taulat 116, 08005, Barcelona, Spain.

Molecular and Cellular Biochemistry
|September 9, 2024
PubMed
Summary
This summary is machine-generated.

This study developed a fast decellularization method for human pericardium tissue, creating a biocompatible scaffold. The resulting acellular matrix preserves extracellular matrix structure and mechanical properties for tissue engineering applications.

Keywords:
DecellularizationHuman acellular matrixPericardial allograftTissue engineeringTissue establishment

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue engineering scaffolds aim to mimic the native extracellular matrix (ECM).
  • Natural materials offer advantages but require processing to ensure biocompatibility.
  • Human pericardium is a potential source for tissue scaffolds.

Purpose of the Study:

  • To develop and validate a rapid decellularization protocol for human pericardium.
  • To assess the biocompatibility and structural integrity of the decellularized pericardium.
  • To evaluate the suitability of the acellular pericardial matrix as a bio-substitute.

Main Methods:

  • Enzymatic and hypertonic decellularization of human pericardium.
  • Histological analysis to confirm cell removal and ECM integrity.
  • Quantification of residual DNA and ECM components (collagen, elastin, glycosaminoglycan).
  • Biomechanical testing to evaluate tensile strength.

Main Results:

  • The decellularization process effectively removed cells while preserving the ECM structure.
  • Residual genetic content was below 50 ng/mg dry tissue.
  • Key ECM biomolecules (collagen, elastin, glycosaminoglycan) were retained.
  • The acellular pericardial matrix maintained its mechanical properties.

Conclusions:

  • The developed super-fast decellularization protocol yields a biocompatible and non-cytotoxic human pericardium graft.
  • The acellular matrix retains essential structural and biomechanical properties of the native ECM.
  • This processed pericardium is suitable for various clinical applications in tissue engineering.