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Identification and validation of coagulation and fibrinolytic-related diagnostic biomarkers for ulcerative colitis by
Feng-Yun Li1, Xue Wu2, Mei-Fang Yao3
1The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou Province, China.
Insights
This study identifies four key genes involved in blood clotting and clot breakdown that could aid in diagnosing ulcerative colitis (UC). These findings offer new avenues for understanding and potentially treating this inflammatory bowel disease.
Area of Science:
- Biomedical research
- Genetics
- Immunology
Background:
- Coagulation and fibrinolysis abnormalities are linked to inflammatory bowel disease.
- Previous research has not systematically investigated the diagnostic potential of coagulation and fibrinolysis genes in ulcerative colitis (UC).
Purpose of the Study:
- To identify diagnostic biomarkers for ulcerative colitis (UC) from coagulation and fibrinolysis-related genes.
- To explore the association of these biomarkers with immune cell infiltration in UC.
Main Methods:
- Utilized UC-related gene expression datasets (GSE169568, GSE94648) from the Gene Expression Omnibus database.
- Applied differential expression analysis and machine learning algorithms to identify biomarkers.
- Performed Gene Set Enrichment Analysis and immune cell infiltration analysis.
Main Results:
- Identified four significant biomarkers: MAP2K1, CREBBP, TAF1, and HP.
- These biomarkers were enriched in immune-related pathways, including T-cell receptor signaling and chemokine signaling.
- Found significant differences in the infiltration of eosinophils, macrophage M0, resting mast cells, and regulatory T cells between UC patients and controls.
- All identified biomarkers showed a significant correlation with eosinophil levels.
Conclusions:
- Discovered four novel coagulation and fibrinolysis-related biomarkers (MAP2K1, CREBBP, TAF1, HP) for ulcerative colitis (UC) diagnosis.
- These biomarkers are associated with immune system pathways and specific immune cell types.
- The findings provide a foundation for further clinical investigation and potential therapeutic strategies for UC.
Abstract:
Abnormalities in coagulation and fibrinolytic status have been demonstrated to be relevant to inflammatory bowel disease. Nevertheless, there is no study to methodically examine the role of the coagulation and fibrinolysis-related genes in the diagnosis of ulcerative colitis (UC). UC-related datasets (GSE169568 and GSE94648) were originated from the Gene Expression Omnibus database. The biomarkers related to coagulation and fibrinolysis were identified through combining differentially expressed analysis and machine learning algorithms. Moreover, Gene Set Enrichment Analysis and immune analysis were carried out. A total of 4 biomarkers (MAP2K1, CREBBP, TAF1, and HP) were identified, and biomarkers were markedly enriched in pathways related to immunity, such as T-cell receptor signaling pathway, primary immunodeficiency, chemokine signaling pathway, etc. In total, the infiltrating abundance of 4 immune cells between UC and control was markedly different, namely eosinophils, macrophage M0, resting mast cells, and regulatory T cells. And all biomarkers were significantly relevant to eosinophils. Our findings detected 4 coagulation and fibrinolysis-related biomarkers (MAP2K1, CREBBP, TAF1, and HP) for UC, which contributed to the advancement of UC for further clinical investigation.
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