[Simultaneous cultivation of the fungi Trichoderma longibrachiatum and Endomycopsis fibuligera]

Combined cultivation of the following microorganisms was studied: the fungus Trichoderma longibrachiatum producing cellulases and the yeast Endomycopsis fibuligera producing glucoamylase. A growth medium was found to maintain the activity of these enzymes at a high level in the both microbial monocultures. The effect of the yeast inoculation time on the enzyme activity was studied during combined cultivation of the two organisms. When the yeast was inoculated during the first two days of the fungal growth, the enzyme activities were 40-70% of those during the growth of the monocultures. The yeast did not grow when it was inoculated by the 4th day of the fungal growth. When the yeast was added to the fungus earlier, the activity of cellulases fell down. Possible reasons for these phenomena are discussed.