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miR-19a-3p promotes the growth of hepatocellular carcinoma by regulating p53/SOX4
Hang Zhang1,2, Jiajun Zhu1,2, Jingjun Zhang3
1Department of Laboratory Medicine, Huashan Hospital, Fudan University, 12 Wulumuqi Middle Road, Shanghai, 200040, China.
Objective:
This study aims to investigate the potential functions of miR-19a-3p in HCC.
Method:
We collected serum samples to analyze miR-19a-3p expression. We utilized CCK8 and Transwell assays to access miR-19a-3p's influence on HCC cells malignancy. We used dual-luciferase reporter and western blotting to validate the impact of p53/miR-19 on miR-19/SOX4.
Results:
The results demonstrated that miR-19a-3p was highly expressed in pre-operative serum samples and HCC cells, which can promote cell proliferation, migration and invasion in HCC under in vitro conditions. Additionally, there was a p53 binding site on the upstream of miR-19a-3p, which was inhibited by p53. SOX4 was the direct gene targeted by miR-19a-3p. The imbalance of p53-miR-19-SOX4 loop was one reason for the progress of HCC.
Conclusion:
Our findings validate the mechanisms of miR-19a-3p and highlight its potential as a therapeutic target in HCC.
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