AuxSynBio: synthetic biology tools to understand and engineer auxin

The plant hormone auxin is a crucial coordinator of nearly all plant growth and development processes. Because of its centrality to plant physiology and the modular nature of the signaling pathway, auxin has played a critical role at the forefront of plant synthetic biology. This review will highlight how auxin is both a subject and an object of synthetic biology. Engineering biology approaches are deepening our understanding of how auxin pathways are wired and tuned, particularly through the creative use of signaling pathway recapitulation in yeast and engineered orthogonal auxin-receptor pairs. Auxin biology has also been mined for parts by synthetic biologists, with components being used for inducible protein degradation systems (auxin-inducible degron), auxin biosensors, synthetic cell-cell communication, and plant engineering.