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Related Concept Videos

Volatilization01:10

Volatilization

374
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
374
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

192
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
192
Sampling Plans01:23

Sampling Plans

169
Sampling is a crucial step in analytical chemistry, allowing researchers to collect representative data from a large population. Common sampling methods include random, judgmental, systematic, stratified, and cluster sampling.
Random sampling is a method where each member of the population has an equal chance of being selected for the sample. It involves selecting individuals randomly, often using random number generators or lottery-type methods. For example, when analyzing the properties of a...
169

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Related Experiment Video

Updated: Jun 13, 2025

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
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Generating pooled quality control samples of volatile organic compounds.

Waqar Ahmed1, Maxim Wilkinson1,2,3, Stephen J Fowler1,4

  • 1Division of Immunology, Immunity to Infection and Respiratory Medicine; School of Biological Sciences; Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.

Journal of Breath Research
|September 11, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for creating pooled quality control (QC) samples for volatile organic compound (VOC) analysis. These QC samples help ensure data quality in complex studies involving breath and headspace VOCs.

Keywords:
GC-MSbreath analysismetabolomicspooled QCquality controlstandardisationthermal desorption

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Area of Science:

  • Analytical Chemistry
  • Biomarker Discovery
  • Metabolomics

Background:

  • Untargeted analysis of volatile organic compounds (VOCs) in exhaled breath and culture headspace is crucial for biomarker discovery.
  • Confounding factors often affect VOC analysis, necessitating robust quality control measures.
  • Existing reference standards may not fully represent the complexity of real-world samples.

Purpose of the Study:

  • To develop and validate a method for generating pooled quality control (QC) samples for untargeted VOC studies.
  • To assess the utility of these pooled QC samples in managing data quality and batch effects.
  • To provide a reliable QC solution for complex VOC matrices like exhaled breath and culture headspace.

Main Methods:

  • A split-recollection workflow using thermal desorption tubes was employed to create pooled QC samples.
  • Individual sample aliquots were desorbed, split, and recollected onto a single QC tube.
  • The pooled QC sample was repeatedly analyzed with varying split ratios to generate multiple injections.

Main Results:

  • Pooled QC samples were demonstrated to be representative of complex VOC mixtures.
  • Principal component analysis confirmed the consistency and reliability of the generated QC samples.
  • The method allows for the injection of multiple QC samples from a single pooled source.

Conclusions:

  • The proposed pooled QC sample generation method offers a valuable tool for untargeted VOC analysis.
  • This approach can enhance data quality and facilitate batch effect correction in longitudinal and multi-center studies.
  • The method is applicable to diverse VOC matrices, including exhaled breath and microbial cultures.