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15-Lipoxygenase in human platelets.

P Y Wong, P Westlund, M Hamberg

    The Journal of Biological Chemistry
    |August 5, 1985
    PubMed
    Summary
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    Human platelets metabolize arachidonic acid, forming 12-hydroxyeicosatetraenoic acid and novel dihydroxy metabolites. This study reveals a 15-lipoxygenase enzyme in platelets, alongside the known 12-lipoxygenase.

    Area of Science:

    • Biochemistry
    • Human Physiology

    Background:

    • Platelets play a crucial role in hemostasis and thrombosis.
    • Arachidonic acid metabolism is central to platelet function and signaling.

    Purpose of the Study:

    • To investigate the metabolic pathways of arachidonic acid in human platelets.
    • To identify novel arachidonic acid metabolites produced by platelets.
    • To characterize the enzymatic activities involved in arachidonic acid metabolism.

    Main Methods:

    • Incubation of washed human platelets with [1-14C]arachidonic acid.
    • Analysis of labeled metabolites using chromatographic techniques (HPLC).
    • Identification of compounds via UV spectroscopy and mass spectrometry of derivatives.

    Main Results:

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    • Extensive conversion of arachidonic acid to 12-hydroxyeicosatetraenoic acid.
    • Formation of minor dihydroxy metabolites: 14,15- and 8,15-dihydroxyeicosatetraenoic acids.
    • Identification of 15-hydroxyeicosatetraenoic acid in the monohydroxy fraction.
    • Bradykinin enhanced the formation of these metabolites.

    Conclusions:

    • Human platelets possess both 12-lipoxygenase and 15-lipoxygenase activities.
    • Novel dihydroxyeicosatetraenoic acid isomers are formed from arachidonic acid in platelets.
    • Bradykinin stimulates the production of these lipoxygenase products.