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Updated: Jun 13, 2025
Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers
Published on: October 24, 2019
Automated, Transferable, and Ethanol-Free Radiosynthesis of [11C]Butanol
Olujide Oyeniran1,2, Linshan Liu1, Confidence Raymond1
1Lawson Research Institute, Saint Joseph's Health Care London, London, Ontario N6C 2R5, Canada.
Abstract:
Cerebral blood flow and blood-brain barrier permeability assessment are crucial hemodynamic parameters to measure under neurological conditions. In conjunction with positron emission tomography (PET), oxygen-15-labeled water has emerged as a gold standard for measuring cerebral perfusion; however, at higher flow rates, [15O]water extraction becomes nonlinear. In such a scenario, freely diffusible [11C]butanol can provide a truer estimate. Radiosyntheses of [11C]butanol reported to date are protracted, are not automated, or require ethanol in the final formulation. By using a flow-based, captive solvent approach on a commercially available radiosynthesizer, we automated and reduced the synthesis time to 28 min. Forgoing cartridge-based purification for an aqueous high-performance liquid chromatography method, we obtained high purity [11C]butanol in ethanol-free phosphate buffered saline in sufficient yields for clinical PET studies. We here report our expedited, automated, and ethanol-free radiosynthesis of [11C]butanol along with preliminary imaging of a porcine subject.
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