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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Related Experiment Video

Updated: Jun 13, 2025

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

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Enhancing Mushroom Freezing Quality Using Microwave-Assisted Technology.

Majid Yousefi Vardanjani1, Nasser Hamdami2, Mohsen Dalvi-Isfahan3

  • 1Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.

Foods (Basel, Switzerland)
|September 14, 2024
PubMed
Summary
This summary is machine-generated.

Microwave-assisted freezing improves button mushroom texture by creating smaller ice crystals. This method shows potential for reducing freeze damage in mushrooms, enhancing their quality after thawing.

Keywords:
energy consumptionmicrowave-assisted freezing (MAF)mushroomsquality attributes

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Area of Science:

  • Food Science
  • Food Engineering
  • Materials Science

Background:

  • Button mushrooms (Agaricus bisporus) are susceptible to freeze damage, affecting texture and quality.
  • Traditional freezing methods can lead to large ice crystal formation, causing cellular damage.
  • Novel freezing techniques are needed to preserve mushroom quality.

Purpose of the Study:

  • To investigate the impact of microwave-assisted freezing on button mushroom quality.
  • To evaluate how different microwave power levels affect ice crystal formation and mushroom attributes.
  • To determine the potential of microwave-assisted freezing for improving frozen mushroom texture.

Main Methods:

  • Button mushrooms were subjected to freezing at four microwave power levels (0, 10, 20, 30%).
  • Quality attributes including ice crystal size, browning index, and textural properties (hardness) were evaluated after thawing.
  • The power ratio between the microwave system and the freezer was analyzed.

Main Results:

  • Increased microwave power resulted in smaller and more uniform ice crystals.
  • The browning index of mushrooms increased with higher microwave power levels.
  • Mushroom hardness increased with elevated microwave power, indicating improved textural properties.
  • The microwave operating system's power ratio to the freezer power was approximately 20% at maximum power.

Conclusions:

  • Microwave-assisted freezing effectively reduces ice crystal size in button mushrooms.
  • This technique has the potential to mitigate freeze damage, leading to superior mushroom texture.
  • The study confirms the viability of microwave-assisted freezing for preserving mushroom quality.