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Methodology Approach for Microplastics Isolation from Samples Containing Sucrose.

Kornelia Kadac-Czapska¹, Beata Bochentyn², Aleksandra Maślarz¹

¹Department of Bromatology, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland.

Molecules (Basel, Switzerland)

|September 14, 2024

Summary

Researchers optimized microplastic (MP) isolation from sugar solutions. Optimal conditions involve 5% HCl at 70°C for 5 minutes, followed by filtration, ensuring efficient recovery of MPs from food for analysis.

Keywords:

digestion filtration identification isolation microplastics polyethylene pre-treatment scanning electron microscopy sucrose µ-Raman spectroscopy

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Separation and Identification of Conventional Microplastics from Farmland Soils

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Published on: March 21, 2025

Area of Science:

Environmental Science
Analytical Chemistry
Food Safety

Background:

Plastic production leads to microplastic (MP) environmental contamination.
Human exposure to MPs occurs mainly through ingestion via contaminated food and beverages.
Efficient isolation of MPs from food matrices is crucial for risk assessment and mitigation.

Purpose of the Study:

To determine optimal conditions for isolating microplastics from sucrose solutions.
To evaluate the impact of temperature, acid type, and concentration on MP isolation efficiency.
To establish a reliable method for MP analysis in food products.

Main Methods:

Testing separation methods using polyethylene particles in sucrose solutions.
Employing chemical digestion with acids and filtration through various membrane filters (nylon, mixed cellulose ester, cellulose acetate) with pore sizes of 0.8 and 10 µm.
Analyzing the effects of temperature and acid parameters using scanning electron microscopy and µ-Raman spectroscopy.

Main Results:

Increased temperature decreased solution viscosity and sucrose adherence to MPs.
Higher acid concentrations accelerated sucrose hydrolysis, aiding MP separation.
Optimal isolation conditions identified: 5% HCl at 70°C for 5 minutes, followed by efficient membrane filtration.
The optimized method demonstrated high MP recovery and fast filtration without altering particle surface properties.

Conclusions:

Established optimal conditions for microplastic isolation from sucrose-based food simulants.
The developed method provides a reliable basis for quantifying microplastic contamination in food.
This research contributes to improving analytical techniques for microplastic analysis in the food industry.