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Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

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Proposing an Affordable Plasma Device for Polymer Surface Modification and Microbial Inactivation.

William Chiappim1, Felipe Vicente de Paula Kodaira1, Gisele Fátima Soares de Castro2

  • 1Laboratory of Plasmas and Applications, Department of Physics, School of Engineering and Sciences, São Paulo State University (UNESP), Guaratinguetá 12516-410, SP, Brazil.

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This study presents an affordable, 3D-printed plasma device for material surface modification and antimicrobial applications. The device effectively reduces microbial load on surfaces while maintaining high cell viability, offering a cost-effective solution.

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Area of Science:

  • Materials Science
  • Biomedical Engineering
  • Plasma Physics

Background:

  • Dielectric barrier discharge (DBD) plasma offers versatile surface modification capabilities.
  • Existing plasma devices can be expensive and complex, limiting accessibility.
  • Atmospheric pressure plasma offers advantages in terms of operational simplicity and cost.

Purpose of the Study:

  • To develop and characterize an affordable, 3D-printed parallel-plate DBD plasma device.
  • To evaluate the device's efficacy in surface modification of polyethylene.
  • To assess the antimicrobial potential against common pathogens and its effect on mammalian cell viability.

Main Methods:

  • A parallel-plate DBD plasma device was designed using 3D printing with a metallic mesh electrode.
  • The device was powered by a modified high-voltage supply and operated using atmospheric air.
  • Surface elemental composition of treated polyethylene was analyzed using X-ray photoelectron spectroscopy (XPS).
  • Antimicrobial efficacy was tested against Candida albicans and Staphylococcus aureus.
  • Vero cell viability was assessed to determine biocompatibility.

Main Results:

  • Plasma treatment of polyethylene for 15 minutes resulted in oxygen incorporation (approx. 15 at%) and a decrease in carbon content (to approx. 80 at%).
  • Over 99% reduction in microbial load for both Candida albicans and Staphylococcus aureus was achieved within 1 to 10 minutes of exposure.
  • Vero cell viability remained high (91-96%) across tested exposure times, indicating good biocompatibility.

Conclusions:

  • The developed 3D-printed DBD plasma device is an affordable and effective tool for material surface modification.
  • The device demonstrates significant antimicrobial potential against relevant pathogens.
  • Its high biocompatibility and cost-effectiveness suggest broad applicability in infection control and material science.