Related Experiment Video
Updated: Jun 13, 2025
MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
Published on: June 24, 2014
Shared and unique transcriptomic signature genes and pathways among biopsy, peripheral blood mononuclear cells and
Seha Akduman1, Nur Ekimci Gürcan1, Nehir Kizililsoley2
1Yeditepe University Faculty of Medicine, İstanbul, Türkiye.
Introduction:
Idiopathic pulmonary fibrosis (IPF) affects the tissue surrounding the alveoli and occurs when the lung tissue becomes thick and stiff for unknown reasons. Clinical findings are fairly well settled, but the molecular mechanisms of IPF are still poorly known.
Materials And Methods:
To further our understanding, we collected publicly available transcriptome dataset from IPF cohorts, grouped them according to sampling method [bronchoalveolar lavage (BAL), biopsy, blood], and performed comparative meta-transcriptome study to (I) unravel key pathways (II), set out differences in discovered genes, pathways, and functional annotation with respect to the sampling method, and (III) find biomarkers for early diagnosis.
Result:
The resulting lists are also compared with DisGeNet reported genes, earlier work, and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Several pathways are shared among BAL and biopsy samples while blood samples point to alternative pathways, indicating the noise in information obtained from these samples.
Conclusions:
Common to all sampling methods, interleukin-10 pathway and extracellular signaling pathways are pointed as further targets.
