Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

819
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
819
  1. Home
  3. Evaluating The Efficacy Of Three Y-strs Commercial Kits In Degraded Skeletal Remains.
  1. Home
  3. Evaluating The Efficacy Of Three Y-strs Commercial Kits In Degraded Skeletal Remains.

Related Experiment Video

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.7K

Evaluating the efficacy of three Y-STRs commercial kits in degraded skeletal remains.

Christian Haarkötter1, María Isabel Medina-Lozano1, Diana C Vinueza-Espinosa1

  • 1University of Granada, Laboratory of Genetic Identification & Human Rights (LABIGEN-UGR), Department of Legal Medicine, Faculty of Medicine, Av. Investigación 11 - PTS - 18016 Granada, Spain.

Science & Justice : Journal of the Forensic Science Society
|September 14, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

This study compared three Y-STR kits for degraded skeletal remains. PowerPlex® Y23 performed best, offering more markers and higher DNA output, crucial for forensic identification.

Keywords:
Degraded human DNADegraded samplesSkeletal remainsY chromosomeY-STRs

More Related Videos

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts
07:56

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

17.5K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.8K

Related Experiment Videos

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

15.7K
Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts
07:56

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

17.5K
Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.8K

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Y chromosome short tandem repeats (Y-STRs) typing is vital for forensic identification, especially in mass grave or disaster victim identification scenarios.
  • Limited comparative studies exist for commercial Y-STR kits, particularly with challenging degraded skeletal samples.

Purpose of the Study:

  • To evaluate and compare the performance of three commercial Y-STR kits: Yfiler™ Plus, PowerPlex® Y23, and Investigator® Argus Y-28.
  • To assess their efficacy on degraded skeletal remains from mass grave contexts.

Main Methods:

  • Analysis of 63 degraded skeletal remains using three commercial Y-STR kits.
  • Performance evaluation based on marker yield, DNA output (RFU), and reliability.
  • Attempted development of a predictive model using quantification and autosomal STR data.

Main Results:

  • PowerPlex® Y23 demonstrated superior performance, yielding more reportable markers and double the average RFU compared to the other kits.
  • Yfiler™ Plus and Investigator® Argus Y-28 showed comparable performance.
  • Investigator® Argus Y-28, new to this sample type, performed well.
  • A predictive model for Y-STR success was not successfully developed.

Conclusions:

  • PowerPlex® Y23 is a highly effective kit for Y-STR typing of degraded skeletal remains.
  • Good Y-STR typing results are achievable with a minimum of 50 pg DNA input or prior successful autosomal STR typing of at least 13 markers.