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Updated: Jun 13, 2025

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Double Monolithic Protocol: The Solution to Fluorescence Limitations
Jacobo Somoza-Román1, Javier Tapia Guadix1, Esther Muñoz-Soto2
1Private Practice, Madrid, Spain.
Objective:
The present paper aims to demonstrate the incorporation of the double monolithic protocol (DMP) into a chairside digital workflow to reproduce the fluorescence properties of natural teeth with chairside monolithic restorations when exposed to different light sources.
Clinical Considerations:
A female patient reporting dissatisfaction with her upper anterior teeth was rehabilitated using seven veneers and a three-element bridge. The DMP was applied to the bridge, which consisted of a primary lithium disilicate framework to which leucite-reinforced glass ceramic veneers were cemented. The fluorescence of the different substrates and ceramic restorations was evaluated throughout the rehabilitation process, under 365 and 405 nm light.
Conclusions:
The DMP allows chairside procedures to be optimized by achieving predictable, mechanically resistant, and esthetic restorations.
Clinical Significance:
The DMP is a new chairside solution for developing ceramic restorations with optimal esthetics and combined mechanical properties, eliminating the need for sintering or glazing procedures.
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