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Related Experiment Videos

Rat monoclonal antibody specific for prostaglandin E structure.

T Tanaka, S Ito, O Hiroshima

    Biochimica Et Biophysica Acta
    |August 22, 1985
    PubMed
    Summary

    Monoclonal antibodies targeting prostaglandin E2 were successfully generated using hybridoma technology. These specific antibodies enable sensitive detection of prostaglandin E, proving valuable for immunoassays.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Molecular Biology

    Background:

    • Prostaglandin E2 (PGE2) plays crucial roles in physiological and pathological processes.
    • Development of specific and sensitive assays for PGE2 is essential for research and diagnostics.
    • Hybridoma technology offers a method for producing targeted antibodies.

    Purpose of the Study:

    • To generate and characterize monoclonal antibodies against prostaglandin E2 (PGE2).
    • To evaluate the specificity and affinity of the developed monoclonal antibodies.
    • To assess the utility of these monoclonal antibodies in enzyme immunoassays.

    Main Methods:

    • Hybridoma technology involving fusion of mouse NS-1 myeloma cells with rat spleen cells immunized with PGE2 conjugates.
    • Characterization of monoclonal antibodies, including subclass determination (IgG2a, IgG2b, IgG2c) and affinity measurements.

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  • Enzyme immunoassay (EIA) utilizing purified monoclonal antibodies for PGE2 detection.

    • Main Results:

    • Six distinct monoclonal antibodies against PGE2 were successfully produced.
    • Antibody affinities ranged from 5.8 x 10^6 to 6.7 x 10^8 M^-1.
    • One antibody demonstrated high specificity for the prostaglandin E structure.
    • Enzyme immunoassay detected as little as 30 pg of prostaglandin E1 and 100 pg of prostaglandin E2.

    Conclusions:

    • Hybridoma technology is a reliable method for producing prostaglandin E-specific antibodies.
    • The generated monoclonal antibodies are valuable reagents for sensitive immunoassays, comparable to radioimmunoassay.
    • These findings support the use of monoclonal antibodies in prostaglandin research and clinical applications.