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Updated: May 23, 2026

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
11:50

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Published on: November 4, 2016

Multiple mechanisms regulate the expression of murine immune response genes

C T Wake, R A Flavell

    Cell
    |September 1, 1985
    PubMed

    The immune response antigens (la antigens) are heterodimeric cell-surface glycoproteins that regulate lymphocyte and macrophage interactions leading to the production of antibody against foreign antigens. The class II genes in the I region of the murine major histocompatibility complex encode the alpha and beta chains of the two known la antigens, I-A and I-E. Molecular cloning of the I region led to the discovery of three DNA regions, A beta 2, psi A beta 3, and E beta 2, which share some nucleotide homology with the classical class II beta genes. In this report we present evidence that A beta 2 is transcribed in spleen and in a cultured B cell hybridoma. A beta 2 mRNA is also found on membrane-bound polysomes, suggesting that it is translated. In contrast to the classical I region genes, A beta 2 transcripts are not induced in macrophage cells by gamma-interferon (gamma-IFN). This difference in sensitivity to gamma-IFN suggests that B cells and macrophages regulate the expression of the class II genes by distinct mechanisms.

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