Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Antibiotic Selection

Antibiotic Selection

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single point mutations in global regulatory genes restore cephalosporin resistance in a low-MIC <i>Enterococcus faecium</i> natural isolate.

Antimicrobial agents and chemotherapy·2026

Same author

Silent Reservoirs: Antibiotic-Resistant <i>Escherichia coli</i> in Autochtonous Portuguese Laying Hens.

Pathogens (Basel, Switzerland)·2026

Same author

Plasmid mutation rates scale with copy number.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Biophysics of Bacterial Colonial Structures and the Occupancy of Microecological Spaces.

Biology·2026

Same author

Abnormal Splicing of <i>GALC</i> Transcripts Underlies Unusual Cases of Krabbe Disease.

Biomedicines·2025

Same author

Unnoticed spread of linear VanA-plasmids in vancomycin-variable Enterococcus faecium strains across different regions: a diagnostics challenge.

The Journal of antimicrobial chemotherapy·2025

Search research articles

Related Experiment Video

Updated: Oct 29, 2025

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

Bacteriocin distribution patterns in Enterococcus faecium and Enterococcus lactis: bioinformatic analysis using a

Ana P Tedim^1,2, Ana C Almeida-Santos^3,4, Val F Lanza⁵

¹Group for Biomedical Research in Sepsis (BioSepsis), Instituto de Investigación Biomédica de Salamanca, Salamanca, Spain.

Applied and Environmental Microbiology

|September 16, 2024

Summary

Multidrug-resistant Enterococcus faecium strains utilize bacteriocins for adaptation. Hospitalized strains show increased bacteriocins linked to antibiotic resistance, aiding survival in healthcare settings.

Keywords:

Enterococcus faecium Enterococcus lactis antimicrobial peptides antimicrobial resistance bacteriocin database bacteriocins plasmids

More Related Videos

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

Related Experiment Videos

Last Updated: Oct 29, 2025

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

Area of Science:

Microbiology
Genomics
Antimicrobial Resistance

Background:

Multidrug-resistant Enterococcus faecium is a significant threat, causing severe infections.
Bacteriocin contribution to E. faecium adaptability is poorly understood.
E. faecium is a known bacteriocin producer within the Bacillota phylum.

Purpose of the Study:

To investigate bacteriocin distribution in E. faecium and Enterococcus lactis genomes.
To develop a precise database for identifying Bacillota bacteriocins.
To explore the link between bacteriocins, antibiotic resistance, and mobile genetic elements.

Main Methods:

Development of a tailored database for 76 Bacillota bacteriocins.
Application of the database to 997 E. faecium and E. lactis genomes.
Computational analysis combined with literature mining and epidemiological data.

Main Results:

A novel database showed high precision in bacteriocin identification.
Distinct bacteriocin profiles were observed between E. faecium and E. lactis.
Hospital-acquired E. faecium strains were enriched in specific bacteriocins (enterocin A, bacteriocins 43, AS5, AS11).
Bacteriocin genes correlated with antibiotic resistance (vancomycin, ampicillin) and specific plasmids.

Conclusions:

Bacteriocins enhance E. faecium adaptability and fitness in nosocomial environments.
Bacteriocin production may aid plasmid maintenance and transmission.
Understanding bacteriocin landscapes can inform strategies against high-risk clones and reveal therapeutic potential.