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Effects of Intrahypothalamic Administration of microRNA Inhibitors and Mimetics on Blood Plasma Biomarkers in the Rat
P M Masliukov1, V V Porseva2, P A Anfimova2
1Yaroslavl State Medical University, Ministry of Health of the Russian Federation, Yaroslavl, Russia. mpm@ysmu.ru.
Abstract:
The effect of let-7a-5p, miR-9a-3p, miR-132-3p, miR-218a-5p microRNA inhibitors and mimetics, when administered into the dorsomedial nucleus of the hypothalamus (DMN), on the markers of age-related changes in blood plasma in 3-month-old and 24-month-old male rats was studied. In the 24-month-old control rats, the content of C-reactive protein (CRP) increased, and the level of myoglobin decreased compared to the 3-month-old animals. After te administration of miRNA inhibitors, the level of CRP significantly increased, and the content of myoglobin decreased, and after the administration of miRNA mimetics, opposite changes were observed. We found no significant differences in the content of somatotropic hormone and testosterone between the control and experimental groups, as well as between the 3-month-old and 24-month-old animals.
Insights
MicroRNA mimetics and inhibitors administered to the rat hypothalamus altered age-related changes in C-reactive protein and myoglobin levels. These microRNAs did not affect somatotropic hormone or testosterone.
Area of Science:
- Molecular biology
- Neuroendocrinology
- Aging research
Background:
- Age-related changes in blood plasma markers are significant.
- MicroRNAs (miRNAs) play crucial roles in regulating gene expression.
- The dorsomedial nucleus of the hypothalamus (DMN) is involved in various physiological processes.
Purpose of the Study:
- To investigate the effect of specific microRNA inhibitors and mimetics on age-related plasma markers.
- To determine the impact of microRNA modulation in the DMN on C-reactive protein (CRP) and myoglobin levels.
- To assess the influence of these microRNAs on somatotropic hormone and testosterone in aging rats.
Main Methods:
- Administration of let-7a-5p, miR-9a-3p, miR-132-3p, and miR-218a-5p microRNA inhibitors and mimetics into the DMN of rats.
- Comparison of plasma marker levels between young (3-month-old) and aged (24-month-old) control rats.
- Analysis of plasma C-reactive protein (CRP), myoglobin, somatotropic hormone, and testosterone levels.
Main Results:
- Aged control rats showed increased CRP and decreased myoglobin compared to young rats.
- MicroRNA inhibitors exacerbated the age-related increase in CRP and decrease in myoglobin.
- MicroRNA mimetics reversed these age-related changes in CRP and myoglobin.
- No significant differences in somatotropic hormone or testosterone were observed across groups or ages.
Conclusions:
- Specific microRNAs in the DMN can modulate key plasma markers associated with aging.
- MicroRNA mimetics show potential for counteracting age-related detrimental changes in CRP and myoglobin.
- The studied microRNAs do not appear to influence somatotropic hormone or testosterone levels in the context of aging.
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