Related Experiment Video
Updated: Jun 13, 2025
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
Correlation analysis of serum Rac1 level with asthma control, airway inflammatory response and lung function in
1Department of Pediatrics, The Third Affiliated Hospital of Nanjing Medical University, The Second People's Hospital of Changzhou, Changzhou, 213000, China.
Insights
Serum Rac1 mRNA levels are lower in children with asthma, correlating with poorer lung function and increased inflammation. Higher Rac1 levels indicate better asthma control and prognosis.
Area of Science:
- Pediatric Pulmonology
- Molecular Biology
- Immunology
Background:
- Asthma is a chronic respiratory disease affecting children worldwide.
- Asthma control, airway inflammation, and lung function are key indicators of disease severity and prognosis.
- Rac1, a Rho family GTPase, plays roles in cellular processes relevant to inflammation and immune responses.
Purpose of the Study:
- To investigate the correlation between serum Rac1 enzyme (Rac1) levels and asthma control, airway inflammatory response, and lung function in asthmatic children.
- To evaluate Rac1 as a potential biomarker for asthma severity and prognosis in pediatric patients.
Main Methods:
- A retrospective analysis of 79 children with asthma, categorized by severity (mild, moderate, severe), compared to 36 healthy controls.
- Serum Rac1 mRNA levels, inflammatory factors (TNF-α, IL-5, IL-6, IL-33), and lung function parameters (FVC, FEV1/FVC, PEF, MMEF) were measured and compared.
- Statistical analysis was used to determine correlations between Rac1 levels, inflammatory markers, lung function, and disease control.
Main Results:
- Asthmatic children exhibited significantly lower serum Rac1 mRNA levels, reduced lung function (FVC, FEV1/FVC, PEF, MMEF), and elevated inflammatory markers (TNF-α, IL-5, IL-6, IL-33) compared to controls.
- Worsening asthma severity was associated with decreased Rac1 levels and lung function, and increased inflammation.
- Improved asthma control correlated with increased Rac1 levels and lung function, and decreased inflammation.
- Rac1 levels showed significant negative correlations with inflammatory markers and positive correlations with lung function parameters.
- Rac1 mRNA levels, FVC, FEV1/FVC, PEF, and MMEF were identified as protective factors, while TNF-α, IL-5, IL-6, and IL-33 were risk factors for asthma prognosis.
Conclusions:
- Children with asthma demonstrate lower serum Rac1 mRNA levels, heightened airway inflammation, and impaired lung function.
- Serum Rac1 mRNA levels may serve as a valuable indicator for assessing asthma control, airway inflammation, lung function, and disease severity in children.
- Rac1 has potential as a biomarker for evaluating the state of illness, treatment efficacy, and prognosis in pediatric asthma.
Objective:
To investigate the correlation between serum Rac1 enzyme (Rac1) level with asthma control, airway inflammatory response and lung function in asthmatic children.
Methods:
A retrospective analysis was performed on 79 children with asthma who were diagnosed and treated in our hospital from June 2020 to January 2023. According to the severity of the disease, the children were divided into mild group (25 cases), moderate group (30 cases) and severe group (24 cases). 36 healthy children who underwent physical examination at the same period in our hospital were selected as the control group. The state of an illness, control level, serum mRNA Rac1, inflammatory factors, and lung function of the children in two groups were compared between the control group and the observation group.
Results:
The Rac1 mRNA levels, forced vital capacity (FVC), forced expiratory volume in one second/FVC (FEV1/FVC), peak expiratory flow (PEF), and maximum mid-expiratory flow (MMEF) in the observation group were significantly lower than these in the control group (P < 0.05). The tumor necrosis factor-alpha (TNF-α), interleukin-5 (IL-5), IL-6, and IL-33 in the observation group were markedly higher than these in the control group (P < 0.05). As the state of an illness worsened, the Rac1 mRNA levels, FVC, FEV1/FVC, PEF, and MMEF gradually reduced (P < 0.05), while the levels of TNF-α, IL-5, IL-6, and IL-33 increased (P < 0.05). As the degree of disease control improved, the Rac1 mRNA levels, FVC, FEV1/FVC, PEF, and MMEF gradually elevated (P < 0.05), and the levels of TNF- α, IL-5, IL-6, and IL-33 showed the opposite trend (P < 0.05). Rac1 was negatively related to the levels of TNF-α, IL-5, IL-6 and IL-33 (P < 0.05), and positively to the levels of FVC, FEV1/FVC, PEF and MMEF (P < 0.001). Rac1 mRNA levels, FVC, FEV1/FVC, PEF and MMEF were protective factors, while TNF-α, IL-5, IL-6 and IL-33 were risk factors for the prognosis of children with asthma (P < 0.05).
Conclusion:
Children with asthma have obviously lower serum Rac1 mRNA levels, higher inflammatory factor levels and lower lung function. Serum Rac1 mRNA level may be associated with better asthma control, lower airway inflammatory response, better lung function and lower disease severity. It has important reference value for the evaluation of the state of an illness, efficacy and prognosis of children with bronchial asthma.
More Related Videos
09:58A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
Published on: April 13, 2010
11:54Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples
Published on: January 21, 2018
Related Concept Videos
01:26Asthma-II: Pathophysiology and Classification
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:
01:29Asthma-I: Introduction
01:24Asthma-III: Symptoms and Complications
Classification of Asthma
01:30Asthma-IV: Diagnostic and Management
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
01:20Asthma: Pathogenesis and Management
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...