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Study of cell deformability by a simple method.

J L Mege, C Capo, A M Benoliel

    Journal of Immunological Methods
    |September 3, 1985
    PubMed
    Summary
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    This study introduces a simpler method to measure cell deformability using centrifugation and latex beads, offering a faster and more accessible alternative to micropipette techniques for immunological research.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biophysics

    Background:

    • Cell deformability is crucial for immune cell functions like migration and engulfment.
    • Traditional methods, such as micropipette aspiration, are complex and require specialized equipment.
    • A need exists for simpler, more accessible techniques to assess cell mechanical properties.

    Purpose of the Study:

    • To develop and validate a simpler method for measuring cell deformability.
    • To compare the novel centrifugation-based method with the established micropipette technique.
    • To assess the impact of centrifugation on cell viability and function.

    Main Methods:

    • Cells were centrifuged in petri dishes on a water cushion.
    • Fixed cells were coated with latex beads for height measurement.

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  • The centrifugation method was compared to micropipette aspiration using cell lines.
  • Experiments were conducted in physiological medium with and without specific inhibitors.

    • Main Results:

    • The centrifugation method provides rapid and accurate determination of cell height.
    • Centrifugation did not impair cell proliferative or phagocytic abilities under tested conditions.
    • The new technique allows examination of many cells, suitable for heterogeneous populations.
    • Results correlated well with the micropipette technique.

    Conclusions:

    • The described centrifugation method is a simpler, faster, and effective alternative for assessing cell deformability.
    • This technique is suitable for both experimental research and clinical diagnostics of phagocyte dysfunction.
    • The method's ability to handle heterogeneous cell populations is a significant advantage.