Bioimaging of thiazolidine-4-one-based new probes, fluorimetric detection of Cu²⁺ "on-off" sensor property, DFT...

Thiazolidinones have been the subject of various research areas for their biological activities, thus they were promising scaffolds to develop new drug agents. A novel thiazolidine 4-one-based fluorescent chemosensor probes PS (thiazolidine) and BO (oxazolidine) were designed and synthesized. Both probes showed specific recognition against Cu²⁺ via a "turn-off" fluorescence response in ACN/H₂O (v/v: 50/50) stock solution (10 mM, pH = 7.0) with a detection limit of (for BO: 1.9 nM and PS: 1.03 nM). Finally, the detection of chemosensory PS and BO showed positive potential for the determination of Cu²⁺ in real food samples, drinking water, and mung beans. The compounds were characterized by diferent chemical and spectroscopic methods. The proposed binding mode for PS and BO with Cu²⁺ was confirmed by DFT calculation, and also they elucidated by bioimaging studies against MCF-7 live cell lines. Additionally, the docking experiment was performed on XylE and hAChE targets.