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Intracellular pH changes during experimental sustained hypercapnia.

E Reichart, F Claudon

    Pflugers Archiv : European Journal of Physiology
    |June 12, 1979
    PubMed
    Summary
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    Rats exposed to carbon dioxide (CO2) showed altered intracellular pH (pHi). Muscle and body pHi diverged after 28 days, suggesting different acid-base responses in non-muscular tissues during prolonged hypercapnia.

    Area of Science:

    • Physiology
    • Acid-Base Balance
    • Environmental Physiology

    Background:

    • Prolonged exposure to elevated carbon dioxide (hypercapnia) affects acid-base balance.
    • Understanding tissue-specific intracellular pH (pHi) regulation during hypercapnia is crucial.

    Purpose of the Study:

    • To investigate the time-dependent changes in intracellular pH (pHi) in different rat tissues during sustained normoxic hypercapnia.
    • To examine the impact of superimposed acute hypercapnia on acid-base disturbances.

    Main Methods:

    • Rats were exposed to controlled fractions of inspired CO2 (FICO2 = 0.08 or 0.16) for 3-28 days under normoxic conditions.
    • Intracellular pH (pHi) was measured in erythrocytes, gastrocnemius muscle, and whole body using the 3H-inulin and 14C-DMO method.
    • Acute hypercapnia (FICO2 = 0.20-0.22) was superimposed on sustained hypercapnia.

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    Main Results:

    • Erythrocyte acid-base disturbances correlated with extracellular acidosis.
    • Muscle and mean body pHi tracked similarly for 9-14 days but diverged after 28 days at FICO2 = 0.08.
    • Acid-base disturbance was more pronounced when acute hypercapnia was added early in the exposure period (day 3).

    Conclusions:

    • Sustained hypercapnia leads to tissue-specific intracellular pH regulation.
    • The non-muscular compartment may exhibit distinct acid-base responses compared to muscle mass during prolonged CO2 exposure.
    • The timing of acute hypercapnia significantly influences the severity of acid-base disturbances.