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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 12, 2025

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

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Published on: June 30, 2017

Cellular Thermal Biology Using Fluorescent Nanothermometers.

Kohki Okabe^1,2, Shingo Sotoma³, Yoshie Harada^4,5,6

¹Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan. okabe@mol.f.u-tokyo.ac.jp.

Advances in Experimental Medicine and Biology

|September 17, 2024

Summary

Cells exhibit temperature fluctuations linked to organelles and functions. Novel fluorescent nanothermometers enable precise intracellular temperature measurements, revealing dynamic cellular thermal landscapes.

Keywords:

Cellular thermogenesis Fluorescent nanodiamond Fluorescent polymeric thermometer Intracellular temperature Nitrogen-vacancy center

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Last Updated: Jun 12, 2025

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Published on: June 30, 2017

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

Published on: April 16, 2017

Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Area of Science:

Cellular Biology
Biophysics
Nanotechnology

Background:

Cells possess mechanisms to detect and react to environmental stressors and temperature shifts, exemplified by heat shock responses and thermosensitive transient receptor potential (TRP) channels.
Recent advancements in nanotechnology have introduced novel fluorescent nanothermometers for precise cellular temperature measurement.

Purpose of the Study:

To investigate spontaneous temperature fluctuations within single cells using advanced nanothermometry.
To explore the relationship between intracellular temperature dynamics and cellular organelles and functions.

Main Methods:

Utilized fluorescent polymeric thermometers and fluorescent nanodiamonds as cutting-edge intracellular thermometry tools.
Measured real-time temperature changes in single cells cultured in vitro and within tissues.

Main Results:

Revealed the occurrence of spontaneous spatiotemporal temperature fluctuations within individual cells.
Demonstrated a correlation between these temperature fluctuations and specific organelles, notably mitochondria.
Established a link between intracellular temperature dynamics and overall cellular and physiological functions.

Conclusions:

Intracellular temperature is not static but exhibits dynamic fluctuations.
These thermal dynamics are closely associated with cellular organelles and physiological processes.
Advanced nanothermometry provides critical insights into the interplay between cellular temperature and function.