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Identification of the novel HLA-B*56:100 allele by next-generation sequencing

Margot Lepage1, Xavier Fournel1, Isabelle Favre-Victoire1

  • 1Histocompatibility Laboratory, Etablissement Français du Sang Auvergne-Rhône-Alpes, Lyon, France.

HLA
|September 18, 2024
PubMed

Abstract:

HLA-B*56:100 differs from HLA-B*56:20:02 by one nucleotide substitution at codon 147.2 in exon 3.

Keywords:
HLAHLA‐B*56:100new allelenext generation sequencing

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

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