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Updated: Jun 12, 2025
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Laboratory-Engineered Glioblastoma Organoid Culture and Drug Screening
Published on: January 10, 2025
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A reference for selecting an appropriate method for generating glioblastoma organoids from the application
1Department of Operating Room, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.
Discover Oncology
|September 18, 2024
Summary
Glioblastoma organoids (GBOs) are valuable for studying glioblastoma stem cells (GSCs) and glioblastoma (GBM). Direct cultivation of tumor tissue is the most common GBO generation method, with broad applications in research.
Area of Science:
- Neuroscience
- Oncology
- Biotechnology
Background:
- Glioblastoma organoids (GBOs) are essential models for glioblastoma (GBM) and glioblastoma stem cell (GSC) research.
- Various methods exist for generating GBOs, influencing their utility in scientific studies.
Purpose of the Study:
- To identify predominant GBO generation methods and their applications.
- To provide a reference for selecting optimal GBO generation techniques for GSC and GBM research.
Main Methods:
- A systematic literature search was conducted across four major databases (PubMed, Embase, Web of Science, Wiley Online Laboratory) from August 2021 to August 2023.
- 42 relevant articles were selected from 295 initial results for analysis of GBO generation and application.
Main Results:
- GBOs are generated from single materials (tumor tissues, cells, gene-edited stem cells) or dual materials (tumor and normal organoids).
- Direct cultivation of GBM cells or tissues is the most prevalent method for GBO generation.
- Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), with silenced P53, NF1, and PTEN via CRISPR/Cas9, are frequently used multifunctional stem cells.
- GBOs derived from direct GBM tissue cultivation show the widest range of applications, including molecular mechanisms, therapy, and culture techniques.
Conclusions:
- Direct cultivation of GBM tissue is the most common and versatile method for generating GBOs.
- This review offers guidance for researchers on selecting appropriate GBO generation methods for studying GSCs and GBM.
