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Updated: Jul 19, 2026
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
Published on: April 11, 2013
A microfluidic paper-based fluorescent sensor integrated with a smartphone platform for rapid on-site detection of
Shuai Liu1, Jingkai Zhao2, Junfeng Wu3
1College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, School of Intelligent Manufacturing, Jiangnan University, Wuxi 214122, China.
Abstract:
A novel approach combing a fluorescent microfluidic paper strip with a portable smartphone-based sensing platform is developed for rapid and sensitive detection of omethoate pesticide. The detection mechanism of the microfluidic paper strip is based on the fluorescence quenching of graphene oxide (GO) toward the cyanine 3 (Cy3)-labeled aptamer (Cy3-APT). Upon exposure to omethoate, the Cy3-APT detaches from the surface of GO, resulting in considerable fluorescence recovery, which can be visualized through the smartphone-based sensing platform. The images are analyzed through a self-developed app embedded with a pretrained convolutional neural network model, achieving a high regression coefficient of 0.9964 at an omethoate concentration range of 0-750 nM. The smartphone-based platform enables rapid on-site detection of omethoate pesticide in real samples within 10 min, with results comparable to those obtained using standard methods. In short, the proposed microfluidic paper-based fluorescent sensor combined with the smartphone-based sensing platform enhances the detection performance toward organophosphorus pesticides.
