Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

747
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
747
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

735
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
735
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

506
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
506
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

707
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
707
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

661
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
661

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Green chemical analysis: main principles and current efforts towards greener analytical methodologies.

Analytical methods : advancing methods and applications·2023
Same author

Flow-injection MS analysis as a simplified approach to pesticide screening in apples.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2023
Same author

Overview of Erasmus+ NETCHEM project: ICT networking for overcoming technical and social barriers in instrumental analytical chemistry education.

Environmental science and pollution research international·2020
Same author

Sorbent-excluding sample preparation method for GC-MS pesticide analysis in apple peel.

Biomedical chromatography : BMC·2019
Same author

Assessment of GC-MS response of selected pesticides in apple matrices related to matrix concentration.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes·2019

Related Experiment Video

Updated: Jun 12, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

8.6K

A Top Solvent-Additive-Ionization Technique Combination for Pesticides Direct Infusion MS Analysis.

Darko Anđelković1, Milica Branković2

  • 1Faculty of Agriculture, University of Nis, Krusevac, Serbia.

Journal of Mass Spectrometry : JMS
|September 19, 2024
PubMed
Summary

This study assessed atmospheric pressure ionization techniques, including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI), for pesticide analysis. It identified optimal solvent-additive combinations for enhanced mass spectrometry sensitivity and detection limits.

Keywords:
APCIHESImass spectrometry

More Related Videos

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis
05:20

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Published on: December 8, 2023

615
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

751

Related Experiment Videos

Last Updated: Jun 12, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

8.6K
Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis
05:20

Avocado Sample Preparation Using the QuEChERS Method with Ammonium Formate for Pesticide Analysis

Published on: December 8, 2023

615
Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

751

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Environmental Science

Background:

  • Atmospheric pressure ionization techniques are crucial for mass spectrometry.
  • Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are leading methods.
  • Optimizing these techniques is vital for sensitive pesticide analysis.

Purpose of the Study:

  • To evaluate the ionization efficiency of ESI and APCI for pesticides.
  • To determine the impact of different organic phases and additives (formic acid, ammonium formate) on ionization.
  • To assess the effect of in-source heating on ionization efficiency for direct infusion mass spectrometry.

Main Methods:

  • Direct infusion mass spectrometry was used for pesticide analysis.
  • Four organic solvent phases were tested, both undoped and doped with formic acid and ammonium formate.
  • In-source heating was applied to modified ESI and APCI setups.

Main Results:

  • Specific solvent-additive combinations significantly influenced ionization efficiency.
  • In-source heating demonstrated potential for improving ionization.
  • The study identified optimal conditions for enhanced sensitivity and detectability.

Conclusions:

  • The findings provide guidance for selecting optimal solvent-additive-source combinations in non-chromatographic mass spectrometry.
  • This research supports improved pesticide analysis with lower detection limits.
  • The results contribute to the advancement of direct infusion mass spectrometry techniques.