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Related Experiment Video

Updated: Jun 12, 2025

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
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Biomimetic Liver Lobules from Multi-Compartmental Microfluidics.

Danqing Huang1, Zhuhao Wu1, Ji Wang1

  • 1Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, 210008, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|September 19, 2024
PubMed
Summary

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Engineered liver lobules using microfluidics show promise for treating liver disease. This novel approach creates biomimetic microcarriers that enhance liver regeneration and reduce damage in rats.

Area of Science:

  • Biomaterials Engineering
  • Regenerative Medicine
  • Microfluidics

Background:

  • Constructing large-scale, 3D biomimetic liver lobules with heterogeneous architecture for hepatic disease treatment is challenging.
  • Natural hepatic lobules possess complex architectures crucial for function and nutrient exchange.

Purpose of the Study:

  • To develop a microfluidic strategy for fabricating 3D biomimetic hepatic lobules with coaxially through-pores.
  • To simulate the heterogeneous architecture and nutrient exchange of natural hepatic lobules for potential liver disease therapy.

Main Methods:

  • A multi-channel microfluidic chip was used with parallel capillaries.
  • Sodium alginate (Alg) was flowed through the central channel, while Ca2+-loaded gelatin methacrylate (GelMA) solutions with hepatocytes, mesenchymal stem cells, and endothelial cells were flowed through surrounding channels.
Keywords:
artificial liverbiomimetichepatic lobulemicrofluidicstissue engineering

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  • In situ gelation formed Alg fibers with heterogeneous, multi-cell-laden GelMA microcarriers featuring coaxially through-pores.

    • Main Results:

    • The fabricated microcarriers mimicked the cord-like structure of hepatic lobules, facilitating nutrient exchange.
    • The spatially anisotropic cell arrangement closely simulated hepatic architecture.
    • Transplantation into rat livers demonstrated increased regeneration and decreased necrosis in damaged livers.

    Conclusions:

    • Microfluidic multi-compartmental microcarriers offer a novel strategy for engineering 3D artificial livers.
    • This approach shows potential for clinical translation in treating liver diseases.
    • The biomimetic design enhances liver function and repair in vivo.