Generation of SOX2^ZsGreen reporter authentic porcine induced pluripotent stem cells

The transcription factor SOX2 plays a crucial role in pluripotency during embryogenesis. In this study, we successfully generated porcine induced pluripotent stem cells (piPSC) by transducing porcine fetal fibroblasts with doxycycline-inducible reprogramming lentiviral vectors. To enhance the utility of these piPSCs, we used a CRISPR/Cas9-based homology-directed repair (HDR) system to introduce a nls-zsGreen reporter in-frame and before the stop codon of the SOX2 coding sequence. The incorporation of the zsGreen reporter offers a novel opportunity for real-time monitoring of SOX2 expression and aiding in the characterization of piPSC lines.