Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Partial isolation and function of the prostacyclin regulating plasma factor.

H Deckmyn, C Zoja, J Arnout

    Clinical Science (London, England : 1979)
    |October 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preliminary evidence of effectiveness of TECAR in lymphedema.

    Lymphology·2019
    Same author

    Deletion of GARP on mouse regulatory T cells is not sufficient to inhibit the growth of transplanted tumors.

    Cellular immunology·2018
    Same author

    High and long-term von Willebrand factor expression after Sleeping Beauty transposon-mediated gene therapy in a mouse model of severe von Willebrand disease.

    Journal of thrombosis and haemostasis : JTH·2017
    Same author

    An open conformation of ADAMTS-13 is a hallmark of acute acquired thrombotic thrombocytopenic purpura.

    Journal of thrombosis and haemostasis : JTH·2017
    Same author

    Amplified endogenous plasmin activity resolves acute thrombotic thrombocytopenic purpura in mice.

    Journal of thrombosis and haemostasis : JTH·2017
    Same author

    Platelet sequestration and activation during GalTKO.hCD46 pig lung perfusion by human blood is primarily mediated by GPIb, GPIIb/IIIa, and von Willebrand Factor.

    Xenotransplantation·2016
    Same journal

    Edaravone Dexborneol regulates the endothelial PPARγ-PON1 axis to inhibit high-cholesterol-induced atherosclerosis.

    Clinical science (London, England : 1979)·2026
    Same journal

    HDAC9 promotes atherosclerosis by suppressing CYP7A1 and impairing hepatic cholesterol excretion.

    Clinical science (London, England : 1979)·2026
    Same journal

    Clusterin ameliorates LPS-induced ARDS by inhibiting the Wnt/β-catenin pathway.

    Clinical science (London, England : 1979)·2026
    Same journal

    Human cytomegalovirus at the maternal-fetal interface: an overview of pathogenesis, defence, and interventions.

    Clinical science (London, England : 1979)·2026
    Same journal

    Cardiometabolic regulation by adipocyte-derived leptin and the brain melanocortin system.

    Clinical science (London, England : 1979)·2026
    Same journal

    Impact of impaired branched-chain amino acid metabolism on kidney disease.

    Clinical science (London, England : 1979)·2026
    See all related articles

    Vascular rings lose prostacyclin production due to peroxide-induced cyclo-oxygenase inhibition. Specific plasma factors, identified as low molecular weight reducing agents, can restore prostacyclin formation and protect against exhaustion.

    Area of Science:

    • Biochemistry
    • Vascular Biology
    • Pharmacology

    Background:

    • Rat aortic rings cease prostacyclin production after prolonged buffer washing, a phenomenon termed 'exhaustion'.
    • This exhaustion is attributed to cyclo-oxygenase inhibition, likely caused by high peroxide concentrations.
    • The cyclo-oxygenase enzyme converts arachidonic acid to prostacyclin, a crucial vasodilator.

    Purpose of the Study:

    • To investigate the mechanism of prostacyclin production exhaustion in rat aortic rings.
    • To identify factors in human plasma that can restore prostacyclin formation in exhausted rings.
    • To elucidate the role of plasma components in protecting vascular prostacyclin synthesis.

    Main Methods:

    • Incubation of rat aortic rings in buffer to induce exhaustion.

    Related Experiment Videos

  • Assessment of prostacyclin production by measuring conversion of substrates.
  • Partial purification and characterization of a prostacyclin-restoring factor from human plasma.
  • Inhibition assays using H2O2 and guaiacol oxidation.

    • Main Results:

    • Prolonged buffer washing led to exhaustion of prostacyclin production, linked to cyclo-oxygenase inhibition by peroxides.
    • Reduced glutathione delayed exhaustion, while H2O2 accelerated it.
    • Incubation with human plasma or plasma filtrate partially restored prostacyclin formation.
    • A low molecular weight (300-400 Da) polar plasma factor was identified that reactivated prostacyclin generation.

    Conclusions:

    • Vascular prostacyclin exhaustion is mediated by peroxide-induced cyclo-oxygenase inhibition.
    • Low molecular weight plasma components act as reducing cofactors for cyclo-oxygenase, prolonging prostacyclin formation.
    • This plasma activity is crucial for protecting the vascular prostacyclin system from exhaustion during persistent irritation.