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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Two-dimensional liquid chromatography (2D-LC) faces orthogonality challenges, particularly for neutral compounds.
  • Online LCxSFC coupling offers complementary separation mechanisms but has faced interface compatibility issues.

Purpose of the Study:

  • To investigate the impact of the interface configuration on online LCxSFC performance.
  • To develop guidelines for optimal interface selection based on mobile phase characteristics.

Main Methods:

  • Evaluation of six different interface configurations for online LCxSFC.
  • Testing with various LC mobile phases and supercritical fluid chromatography (SFC) conditions.
  • Analysis of lignin monomers and industrial polymers to demonstrate applicability.

Main Results:

  • Empty loops eliminated the need for trapping columns across different LC mobile phases.
  • Successful injection in partial fill mode without CO2 bubble or miscibility issues.
  • Configurable interfaces allowed flexibility comparable to LCxLC systems.

Conclusions:

  • Interface design significantly impacts online LCxSFC performance and flexibility.
  • Guidelines for interface selection facilitate easier implementation of LCxSFC.
  • The optimized online LCxSFC is suitable for routine industrial analysis.