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Margaux Sanchez^1,2, Julien Crepier², Karine Faure¹

¹Universite Claude Bernard Lyon 1, ISA UMR 5280, CNRS, 5 rue de la Doua, 69100 Villeurbanne, France.

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Summary

New online LCxSFC valve technology improves analysis time by enabling flow splitting in the "CO2 stream" configuration. This modification prevents sample loss and enhances performance for complex separations.

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Area of Science:

Analytical Chemistry
Chromatography

Background:

Online liquid chromatography-supercritical fluid chromatography (LCxSFC) interfaces are crucial for enhanced separations.
Existing valve configurations improve peak shape but struggle to reduce analysis time.
Flow splitting is a known technique to reduce analysis time in LCxLC but faces challenges in the CO2 stream configuration of LCxSFC.

Purpose of the Study:

To investigate the feasibility of implementing flow splitting in the
CO2 stream
LCxSFC configuration.
To overcome limitations preventing split integration in this specific configuration.
To enhance the efficiency and reduce analysis time of online LCxSFC methods.

Main Methods:

Development of a modified LCxSFC interface incorporating a second valve.
Implementation of controlled CO2 depressurization upstream of the transfer loop filling phase.
Testing the modified interface with a real sample application, including repeatability tests.

Main Results:

Successful integration of a flow split in the
CO2 stream
LCxSFC configuration.
Prevention of gas escape and sample loss during the transfer process.
Demonstrated high performance and repeatability for real sample analysis.

Conclusions:

The modified LCxSFC interface effectively enables flow splitting in the
CO2 stream
configuration.
This advancement significantly reduces analysis time without compromising sample integrity.
The new interface offers a practical solution for more efficient online LCxSFC separations.