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Updated: Jun 12, 2025

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
Published on: February 23, 2017
A Dual Membrane Electrodialytic Phosphoric Acid Eluent Generator for Ion Exclusion Chromatography-A Gas-Free
Xiaolu Sun1, Jingwen Tang1, Weiqing Chen1
1Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Abstract:
Relative to suppressed conductometric anion exchange chromatography, conductometric ion exclusion chromatography (IEC) is much less popular. Poor limit of detection and manual preparation of eluent are primarily responsible for this. We describe an electrodialytic phosphoric eluent generator for the online production of phosphoric acid eluent at a concentration exceeding 35 mM. It is a sandwiched configuration consisting of three channels isolated by stacked anion exchange membranes and a bipolar membrane plus stacked anion exchange membrane. Such a unique configuration ensures the production of gas-free eluent, obviating the need for a gas removal device. The resulting eluent concentration is controlled by varying the current. Coupled with an ultraviolet absorbance detector, a 0.04-16.67 mg/L limit of detection for model organic acids was achieved by IEC equipped with an electrodialytic phosphoric eluent generator.
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