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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
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Serum cytokine panels in pediatric clinical practice
Paul M Gallo1, Jihwan Kim1, Kevin O McNerney2
1Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, Pa; Immune Dysregulation Program, Children's Hospital of Philadelphia, Philadelphia, Pa.
The Journal of Allergy and Clinical Immunology
|September 20, 2024
Summary
Multiplexed cytokine panels can help differentiate serious pediatric conditions like familial hemophagocytic lymphohistiocytosis (fHLH) and cytokine release syndrome (CRS) from sepsis, guiding clinical decisions.
Area of Science:
- Immunology
- Biomarker Discovery
- Pediatric Critical Care
Background:
- Cytokines are key soluble signaling proteins regulating inflammation and immune responses.
- Serum cytokine panels are increasingly utilized in clinical practice.
- Understanding cytokine profiles as disease biomarkers remains an evolving area.
Purpose of the Study:
- To analyze the real-world application of a multiplexed cytokine panel in a single pediatric center.
- To correlate cytokine panel results with patient diagnosis and disease severity.
- To explore the utility of cytokine panels in pediatric clinical practice.
Main Methods:
- Implementation and validation of a same-day multiplexed cytokine panel assay.
- Retrospective analysis of coded patient data collected via REDCap.
- Correlation of cytokine levels with clinical outcomes and diagnoses.
Main Results:
- Cytokine levels directly correlate with the acuity of care, with highest levels in pediatric intensive care unit patients.
- Distinct cytokine profiles were observed for familial hemophagocytic lymphohistiocytosis (fHLH), sepsis, and cytokine release syndrome (CRS).
- A specific cytokine ratio ([IFN-γ] + [IL-10]) / ([IL-6] + [IL-8]) effectively distinguished fHLH and CRS from severe sepsis.
Conclusions:
- Cytokine panels correlate with illness severity and aid in differentiating similar-presenting syndromes like fHLH, CRS, and sepsis.
- Cytokine panels serve as valuable biomarkers for informing diagnosis and management strategies.
- Further research is needed to fully elucidate complex clinical patterns revealed by cytokine profiling.
