Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relation between serum opsonic activity for Streptococcus pneumoniae and complement function in sickle cell disease.

A B Bjornson, J S Lobel, K S Harr

    The Journal of Infectious Diseases
    |October 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Interleukin-6-associated laboratory parameters and immunohistochemistry in symptomatic stage A and B nodular sclerosing Hodgkin's disease in children.

    Annals of clinical and laboratory science·1997
    Same author

    Serotype-specific immunoglobulin G antibody responses to pneumococcal polysaccharide vaccine in children with sickle cell anemia: effects of continued penicillin prophylaxis.

    The Journal of pediatrics·1996
    Same author

    The pentameric structure of IgM is necessary to enhance opsonization of Bacteroides thetaiotaomicron and Bacteroides fragilis via the alternative complement pathway.

    Microbial pathogenesis·1995
    Same author

    Intracranial neuroblastoma.

    Medical and pediatric oncology·1995
    Same author

    The alternative complement pathway promotes IgM antibody-dependent and -independent adherence of Bacteroides to polymorphonuclear leukocytes through CR3 and CR1.

    Journal of leukocyte biology·1994
    Same author

    Down-regulation of chemotaxis of polymorphonuclear leukocytes following thermal injury involves two distinct mechanisms.

    The Journal of infectious diseases·1993
    Same journal

    NET-inducing ability of Cutibacterium acnes clinical isolates is associated with pathogenicity in acne vulgaris.

    The Journal of infectious diseases·2026
    Same journal

    Optimizing the Use of Proviral DNA HIV Drug Resistance Testing: Clinical Applications and Cautions.

    The Journal of infectious diseases·2026
    Same journal

    Monitoring HLA-A2-restricted T cell responses and BCLA-specific serostatus during human latent Toxoplasma gondii infection suggests the implication of CD8+ T cells in parasite containment.

    The Journal of infectious diseases·2026
    Same journal

    Cryptosporidiosis in Ptients with Inborn Errors of Immunity: Retrospective cohort study of the French National Reference Center (CEREDIH).

    The Journal of infectious diseases·2026
    Same journal

    Type 3 fimbrial regulation underpins anti-MrkA immunotherapeutic efficacy in experimental Klebsiella pneumoniae infection.

    The Journal of infectious diseases·2026
    Same journal

    Rationalising heterogeneity in Staphylococcus aureus bacteraemia: current progress and future goals.

    The Journal of infectious diseases·2026
    See all related articles

    Children with sickle cell disease show reduced opsonization against Streptococcus pneumoniae, impacting complement pathways. This may increase pneumococcal bacteremia risk due to auxiliary serum factors, not intrinsic complement defects.

    Area of Science:

    • Immunology
    • Hematology

    Background:

    • Sickle cell disease (SCD) is linked to increased infection risk, particularly from encapsulated bacteria like Streptococcus pneumoniae.
    • The complement system, including classic and alternative pathways, is crucial for opsonization and bacterial clearance.

    Purpose of the Study:

    • To investigate complement-mediated opsonization of Streptococcus pneumoniae in children with SCD.
    • To assess the roles of classic and alternative complement pathways and C3 fixation in SCD patients.

    Main Methods:

    • Sera from children with SCD and healthy siblings were used to measure opsonic activity against specific Streptococcus pneumoniae serotypes.
    • Opsonization was assessed by measuring bacterial uptake and intracellular killing by polymorphonuclear leukocytes.
    • Complement component 3 (C3) fixation via classic and alternative pathways was quantified using radioimmunoassay.

    Related Experiment Videos

    Main Results:

    • A subset of SCD patients exhibited significantly reduced opsonic activity for both classic and alternative complement pathways.
    • Reduced classic pathway opsonic activity correlated with decreased C3 fixation via this pathway.
    • Alternative pathway C3 fixation remained normal despite reduced opsonic activity, suggesting involvement of auxiliary serum factors.

    Conclusions:

    • Opsonic defects in SCD patients against Streptococcus pneumoniae may stem from auxiliary serum factors rather than intrinsic complement system abnormalities.
    • Reduced opsonic activity, particularly via the classic pathway, is associated with an increased risk of pneumococcal bacteremia in SCD.
    • Further research into these auxiliary factors is warranted to understand and mitigate infection risk in SCD.