Complex In Silico RNA Design with MoiRNAiFold

In the advent of the RNA therapeutics and diagnostics era, it is of great relevance to introduce new and more efficient RNA technologies that prove to be effective tools in practical contexts. Moreover, it is of utmost importance to develop and provide access to computational tools capable of designing such RNA constructs. Here we introduce one such novel diagnostics technology (Apta-SMART) and show how to design (using MoiRNAiFold) and implement it, step by step. Moreover, we show how to combine this technique with well-known RNA amplification methods and briefly mention some encouraging results.