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Double-stranded DNA antibody test using BioPlex220 system: unacceptable option for SLE diagnosis and follow-up
Kamran Kadkhoda1,2, Gordon Schroeder1, Nicole Boyert1
1Immunopathology Laboratory, Robert J. Tomsich Pathology & Laboratory Medicine Department, Diagnostics Institute, Cleveland Clinic, Cleveland, OH, US.
Abstract:
The anti-double-stranded (ds)DNA antibody test is an integral part of diagnosing systemic lupus erythematosus when the entry criterion is satisfied. We investigated the sensitivity of the BioPlex 2200 instrument compared with the serological gold standard and other tests and clinical information. The results showed an unacceptable sensitivity for this method. Laboratories should be cognizant of this shortcoming when selecting this platform for dsDNA antibody testing.
