Impact of egg white protein on mycoprotein gel: Insights into rheological properties, protein structure and Molecular...

The mechanisms behind Mycoprotein (MYC) formation, especially the interactions with egg white proteins (EWP), are not fully understood despite the widespread use of fungal proteins. This study investigates the formation of MYC-EWP composite gels using physicochemical and structural analyses, supported by molecular simulations. At pH 7, EWP adsorbs onto the MYC mycelium surface via hydrogen bonding and electrostatic interactions, with a binding energy of -21.97 kcal/mol. Increasing EWP concentration enlarges particle size in the suspension. Upon heating, the gel's microstructure shows irregular fibrous structures with EWP filling the spaces within the mycelium. The texture properties improve with higher EWP levels, resulting in a denser gel structure. These findings offer key insights into MYC-EWP interactions, clarifying the gelation process and providing valuable guidance for optimizing fungal protein products.