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Updated: Jun 12, 2025
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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
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Dermal fibroblasts retain site-specific transcriptomic identity in keloids
Pingping Lin1, Daoning Zhang1, Jie Tian2
1Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China; National Clinical Research Center for Skin and Immune Diseases, Beijing, China; Beijing Key Laboratory of Molecular Diagnosis on Dermatoses, Beijing, China; NMPA Key Laboratory for Quality Control and Evaluation of Cosmetics, Beijing, China.
Journal of Dermatological Science
|September 25, 2024
Summary
Keloids retain skin's positional identity and spatial differences across body sites. Fibroblasts are key to this heterogeneity, even in lab conditions, showing developmental imprinting.
Area of Science:
- Dermatology
- Genomics
- Cell Biology
Background:
- Human skin exhibits significant spatial heterogeneity and positional identity.
- The influence of disease on these site-specific differences is unclear, particularly in keloids.
Purpose of the Study:
- To determine if spatial heterogeneity and positional identity persist in keloids.
- To investigate the role of cell types in maintaining keloid positional identity.
Main Methods:
- Transcriptome sequencing of 139 keloid tissues and 19 fibroblast samples from seven anatomic sites.
- Single-cell RNA sequencing to analyze cell type contributions to positional identity.
Main Results:
- Keloids maintained distinct, anatomically-related gene expression profiles across different body regions.
- Regional differences in keloids exceeded variations between donors.
- Mesenchymal cells, especially fibroblasts, were crucial for keloid positional identity.
- Fibroblast positional identity persisted even after extended in vitro culture.
Conclusions:
- Keloids exhibit positional identity and developmental imprinting similar to normal skin.
- Fibroblasts are the primary drivers of spatial heterogeneity in keloids.
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