Multiple Biomarker Responses in Aegla castro Exposed to Copper: A Laboratory Approach

Although some biomarkers have already been determined in aeglids collected in the field, data from laboratory exposures are scarce. To our knowledge, no studies have investigated oxidative stress biomarkers in aeglids exposed to metals in the laboratory, or performed hemocyte counts and the comet assay using gill and hepatopancreas of aeglids. Thus, we investigated the effects of acute Cu exposure on intermolt males of Aegla castro, collected from a reference stream, acclimated for 6 days in the laboratory, and then exposed to 11 μg L^-1 of dissolved Cu (Cu 11) or only to water (CTR), for 24 h. Gill and hepatopancreas samples were used to determine Cu accumulation, DNA damage, and metallothionein content (MT), while hemolymph samples were used to determine Cu accumulation, DNA damage, and hemocyte counts. Muscle samples were used to determine Cu accumulation and acetylcholinesterase activity (AChE). Non-protein thiol content (NPSH), catalase (CAT), glutathione S-transferase activities (GST), lipoperoxidation (LPO), and protein carbonylation content (PCC) were measured only in the hepatopancreas. Aegla castro exposed to Cu accumulated this metal in gills and activated detoxification mechanisms, through increased MT content in the gill, and showed an immune response, evidenced by an increase in hyaline hemocytes. Therefore, gill and hemocytes appear to have a protective role in preventing the transport and bioavailability of Cu through the body. On the other hand, we observed a decrease in MT content in the hepatopancreas of crabs exposed to Cu, suggesting the excretion of MT in association with Cu bound to the sulfhydryl groups of this protein.