Single-cell detection and linear discriminant analysis of bacterial Raman spectra in glass filter microholes

We report a study of micro-Raman spectroscopy towards an optimal approach for single cell measurements for the detection of bacteria by vibrational spectroscopy. The use of glass membrane filters was tested by microfiltration to separate individual bacterial cells. The glass membrane filters were applied to the study of Raman spectral classification analysis. This approach achieved the capture and individual detection of spiked bacterial cells. Linear discriminant analysis (LDA) of Raman spectra measured on glass membrane filters was successfully used to distinguish several bacterial species.