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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jul 7, 2026

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Using gold-based nanomaterials for fighting pathogenic bacteria: from detection to therapy.

Jia-Ni Man¹, Jian Zhu², Guo-Jun Weng¹

¹The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.

Mikrochimica Acta

|September 26, 2024

Summary

Gold nanomaterials (GNMs) offer unique properties for pathogen detection and antimicrobial applications. This review highlights their use in sensitive pathogen sensing and effective elimination strategies.

Keywords:

Antimicrobial strategies Biosensors Gold-based nanomaterials Optical detection Pathogen

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Area of Science:

Nanomaterials Science
Biotechnology
Analytical Chemistry

Background:

Gold nanomaterials (GNMs) exhibit unique quantum size and surface effects.
GNMs show significant potential for pathogen detection and broad-spectrum antimicrobial activity.

Purpose of the Study:

To review recent advancements in using GNMs for pathogen detection.
To summarize the application of GNMs in pathogen elimination.
To explore integrated pathogen detection and treatment strategies using GNMs.

Main Methods:

Overview of physicochemical properties of gold nanomaterials.
Discussion of various detection strategies including colorimetric, fluorescence, surface-enhanced Raman scattering (SERS), dark-field microscopy, and electrochemical methods.
Exploration of antimicrobial applications such as agent delivery, photothermal conversion, and synergistic effects with photosensitizers.

Main Results:

GNMs enable efficient, sensitive, and specific pathogen detection through diverse analytical techniques.
GNMs demonstrate broad-spectrum antimicrobial activity via multiple mechanisms.
Integrated approaches combining detection (e.g., colorimetric, SERS) with treatment (e.g., photothermal sterilization) are feasible.

Conclusions:

GNMs are versatile tools for both detecting and eliminating pathogens.
Future research should focus on further optimizing GNMs for integrated diagnostic and therapeutic applications.
The unique properties of GNMs position them as key players in combating infectious diseases.