Advancements in reliability of mechanical performance of 3D PRINTED Ag-doped bioceramic antibacterial scaffolds for...

The current gold-standard approach for addressing bone defects in load-bearing applications sees the use of either autographs or allographs. These solutions, however, have limitations as autographs and allographs carry the risk of additional trauma, the threat of disease transmission, and potential donor rejection. An attractive candidate for overcoming the challenges associated with the use of autographs and allographs is a 3D porous scaffold displaying the needed mechanical competency for use in load-bearing applications that can stimulate bone tissue regeneration and provide antibacterial capabilities. To date, no reports document a 3D porous scaffold that fully meets the criteria specified above. In this work, we show how the use of fused filament fabrication (FFF) 3D printing technology in combination with a bimodal distribution of Ag-doped bioactive glass-ceramic (Ag-BG) micro-sized particles can successfully deliver porous 3D scaffolds with attractive and reliable mechanical performance characteristics capable of stimulating bone tissue regeneration and the ability to provide inherent antibacterial properties. To characterize the reliability of the mechanical performance of the FFF-printed Ag-BG scaffolds, Weibull statistics were evaluated for both the compressive (N = 25; m = 13.6 ± 0.9) and flexural (N = 25; m = 7.3 ± 0.7) strengths. Methicillin-resistant Staphylococcus aureus (MRSA) was used both in planktonic and biofilm forms to highlight the advanced antibacterial characteristics of the FFF-printed Ag-BG scaffolds. Biological performance was evaluated in vitro through indirect exposure to human marrow stromal cells (hMSCs), where the FFF-printed Ag-BG scaffolds were found to provide an attractive environment for cell infiltration and mineralization. Our work demonstrates how fused filament fabrication technology can be used with bioactive and antibacterial materials such as Ag-BG to deliver mechanically competent porous 3D scaffolds capable of stimulating bone tissue regeneration while simultaneously providing antibacterial performance capabilities.