Related Experiment Video
Updated: Jun 12, 2025
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Systemic ozone therapy as an adjunctive treatment in guided bone regeneration: a histomorphometrical and
Ana Maira Pereira Baggio1, Vinícius Ferreira Bizelli2, Izabela Fornazari Delamura2
1Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), São Paulo, Brazil. ana.baggio@unesp.br.
Objectives:
To assess the effectiveness of ozone therapy in guided bone regeneration (GBR) for critical size calvarial defects in rats.
Materials And Methods:
96 male Wistar rats were divided into four groups (n = 6 each). An 8 mm critical defect was created in the calvaria of each rat. The groups were: BIO (porcine collagen membrane, BioGide®), BIO + OZ (membrane with systemic ozone therapy every 2 days), COA + OZ (blood clot with ozone therapy), and COA (blood clot only). Evaluations at 7, 15, 30, and 60 days included histological, histomorphometric, inflammatory profile, Micro-CT, and immunohistochemical analyses. Statistical analysis involved two-factor ANOVA with Tukey's post-hoc test for general data, and one-factor ANOVA with Holm-Sidak post-hoc test for Micro-CT data.
Results:
The BIO + OZ group demonstrated superior bone regeneration with well-organized, mature bone tissue and significant bone formation at 30 and 60 days. The COA + OZ group showed early angiogenesis and reduced inflammation, resulting in complete defect closure by 30 days. The BIO group had good regeneration, but less mature tissue compared to BIO + OZ. The COA group exhibited limited bone formation and higher porosity.
Conclusion:
Ozone therapy positively influences bone regeneration by enhancing cell proliferation and the healing response.
Clinical Relevance:
Improving regenerative processes with auxiliary therapies like ozone therapy can be significant for advancing dental reconstructions.
