Optimizing Extraction, Evaluating Antioxidant Activity, and Analyzing Bioactive Compounds in Trikaysornmas Formula.

Area of Science:

• Herbal Medicine
• Phytochemistry
• Pharmacology

Background:

• Trikaysornmas formula (TKM) is a traditional Thai remedy with uses including appetite enhancement, tonic, and adaptogenic properties.
• The formula comprises Aegle marmelos fruit, Nelumbo nucifera stamen, and Jatropha multifida bark.
• Understanding TKM's composition and activity is crucial for its standardization and therapeutic potential.

Purpose of the Study:

• To optimize the extraction method for TKM.
• To identify and quantify active compounds within the TKM.
• To evaluate the antioxidant capacity of TKM using various assays.

Main Methods:

• Extraction method optimization using experimental design.
• Phytochemical analysis including total phenolics, flavonoids, carotenoids, and alkaloids via colorimetric methods.
• Antioxidant activity assessment using DPPH, ABTS, ORAC, FRAP, metal chelating, and lipid peroxidation assays.
• Quantification of specific active constituents (gallic acid, kaempferol-3-o-glucoside, imperatorin, vitexin, scopoletin) using a validated High-Performance Liquid Chromatography (HPLC) method.

Main Results:

• Optimized extraction parameters were established for TKM.
• The formula contains significant levels of total phenolics, flavonoids, carotenoids, and alkaloids.
• TKM exhibited notable antioxidant activities across multiple assays, including free radical scavenging and reducing power.
• The developed HPLC method demonstrated reliability for analyzing active constituents.

Conclusions:

• The study successfully optimized TKM extraction and validated an HPLC method for active compound analysis.
• TKM possesses significant antioxidant properties, supporting its traditional uses.
• Findings provide a basis for TKM standardization, quality control, and exploration of its therapeutic applications.