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Updated: Jun 12, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

Maximizing single cell dissociation protocol for individual zebrafish embryo.

Shruthi VijayKumar¹, Michael Borja¹, Norma Neff¹

¹Chan Zuckerberg Biohub, San Francisco, USA.

|September 27, 2024

Summary

This summary is machine-generated.

We optimized a single-cell isolation protocol for zebrafish embryos, enabling detailed studies of cellular heterogeneity even with limited starting material. This method supports research into developmental biology and disease mechanisms.

Keywords:

Dissociation Single zebrafish embryo dissociation Single-cell Zebrafish

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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

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Last Updated: Jun 12, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

Area of Science:

Developmental Biology
Genomics
Cell Biology

Background:

Single-cell sequencing is crucial for understanding cellular heterogeneity and cell states.
Challenges exist in preparing high-quality single-cell suspensions, especially from limited samples like zebrafish embryos.
Zebrafish embryos are vital models for developmental biology and disease research.

Purpose of the Study:

To present an optimized protocol for isolating single cells from individual zebrafish embryos.
To provide a resource for researchers working with limited starting material.
To enable unique investigations using single zebrafish embryos.

Main Methods:

Refined single-cell isolation protocol.
Utilized zebrafish embryos as the model organism.

Main Results:

Successfully optimized protocol for isolating single cells from individual zebrafish embryos.
Protocol facilitates unique investigations such as inter-individual genetic differences and embryo-specific lineage tracing.
Provides a valuable resource for researchers with limited starting material.

Conclusions:

The optimized protocol enhances the utility of zebrafish embryos for single-cell studies.
Enables exploration of cellular emergence, types, and states.
Advances understanding of cellular processes and disease mechanisms in developmental contexts.