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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Jun 12, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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Perspectives Toward an Integrative Structural Biology Pipeline With Atomic Force Microscopy Topographic Images.

Jean-Luc Pellequer1

  • 1Univ. Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale (IBS), Grenoble, France.

Journal of Molecular Recognition : JMR
|September 27, 2024
PubMed
Summary

Integrative structural biology combines multiple techniques to model large protein assemblies. Atomic Force Microscopy (AFM) data is now interpretable for these models thanks to new pipelines, overcoming previous limitations.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Recent advances in cryo-electron microscopy and AlphaFold have enabled high-resolution protein structure determination.
  • Complex macromolecular assemblies present challenges beyond the scope of single biophysical techniques.
  • Integrative structural biology aims to combine data from diverse methods for comprehensive modeling.

Purpose of the Study:

  • To review the historical development and current status of Atomic Force Microscopy (AFM) in structural biology.
  • To address the limitations of AFM data interpretability that previously hindered its integration into modeling platforms.
  • To highlight the potential of AFM as a complementary technique in integrative structural biology.

Main Methods:

  • Review of historical AFM applications in structural biology.
  • Examination of AFM imaging strengths and limitations.
  • Discussion of AFM data correction, improvement, and the AFM-Assembly pipeline.
  • Analysis of challenges for integrating AFM into integrative modeling platforms.

Main Results:

  • AFM topographic data, once lacking interpretability, can now be integrated into modeling platforms via tools like AFM-Assembly.
  • Single-molecule techniques like AFM offer advantages in validating models and capturing protein flexibility.
  • Improvements in AFM data processing are crucial for its broader adoption in structural biology.

Conclusions:

  • Atomic Force Microscopy is becoming a valuable tool for integrative structural biology.
  • Overcoming interpretability issues allows AFM to contribute to the structural modeling of large, complex biological assemblies.
  • Further development is needed to fully leverage AFM data within integrative modeling frameworks.