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Deep Immunoprofiling of Large-Scale Tuberculosis Dataset at Single Cell Resolution Reveals a CD81bright γδ T Cell
Mojtaba Shekarkar Azgomi1,2, Giusto Davide Badami1,2, Miriam Di Caro1,2
1Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), Azienda Ospedaliera Universitaria Policlinico (A.O.U.P.) Paolo Giaccone, University of Palermo, 90127 Palermo, Italy.
Cells
|September 27, 2024
Summary
Tuberculosis (TB) involves distinct γδ T cell subsets. CD81 expression on these cells is higher in latent TB infection, suggesting a role in immune response and potential therapeutic targets.
Area of Science:
- Immunology
- Infectious Diseases
Background:
- Tuberculosis (TB) is a major global infectious disease.
- γδ T cells are implicated in immune responses to Mycobacterium tuberculosis.
Purpose of the Study:
- To investigate distinct γδ T cell subsets in TB.
- To identify molecular markers associated with TB protection or progression.
Main Methods:
- Integrated large-scale scRNA-seq and bulk RNA-seq data.
- Defined phenotypic and molecular characteristics of peripheral blood γδ T cells.
- Validated findings using flow cytometry.
Main Results:
- Identified five unique γδ T cell subclusters with distinct functions.
- Discovered a γδ T cell cluster enriched in TCR signaling with high CD81 expression.
- CD81 expression was significantly higher on γδ T cells in latent TB infection (TBI) versus active TB (ATB).
Conclusions:
- Distinct γδ T cell subsets play roles in TB immunity.
- CD81 is a potential biomarker for distinguishing TBI from ATB.
- CD81 signaling pathways may offer therapeutic targets for TB management.
Keywords:
CD81Mycobacterium tuberculosislatent Mycobacterium tuberculosis infectionsingle-cell RNA sequencetuberculosisγδ T cells
